Table 1.

General linear regression model indicating estimated differences, 95% confidence intervals and P-values.

Estimated difference (95% confidence interval) in the number of identified unique peptides
Number of fractions	OFFGEL vs. SDS-PAGE	P-value*	hpRP-HPLC vs. SDS-PAGE	P-value	hpRP-HPLC vs. OFFGEL	P-value
12	−114.6 (−561.8, 332.7)	0.616	967.5 (550.0, 1385.1)	<0.001	1082.1 (588.7, 1575.)	<0.001
20	401.9 (−33.6, 837.4)	0.070	1276.2 (925.6, 1626.7)	<0.001	874.2 (424.4, 1324.0)	<0.001
24	660.2 (81.7, 1238.6)	0.025	1430.5 (1059.8, 1801.1)	<0.001	770.3 (186.4, 1354.2)	0.010
40	N.A.**		2047.7 (1366.9, 2728.4)	<0.001	N.A.**

Estimated difference (95% confidence interval) in the number of identified unique proteins (one peptide)
Number of fractions	OFFGEL vs SDS-PAGE	P-value	hpRP-HPLC vs. SDS-PAGE	P-value	hpRP-HPLC vs. OFFGEL	P-value
12	93.3 (27.0, 159.6)	0.006	121.6 (59.8, 183.5)	<0.001	28.3 (−44.8, 101.4)	0.448
20	121.0 (56.5, 185.6)	<0.001	141.7 (89.8, 193.7)	<0.001	20.7 (−45.9, 87.3)	0.543
24	134.9 (49.2, 220.6)	0.002	151.8 (96.9, 206.7)	<0.001	16.9 (−69.6, 103.4)	0.702
40	N.A.**		192.0 (91.2, 292.9)	<0.001	N.A.**

Estimated difference (95% confidence interval) in the number of identified unique proteins (two or more peptides)
Number of fractions	OFFGEL vs SDS-PAGE	P-value	hpRP-HPLC vs. SDS-PAGE	P-value	hpRP-HPLC vs. OFFGEL	P-value
12	79.1 (23.5, 134.7)	0.005	90.5 (38.6, 142.4)	0.001	11.4 9–49.9, 72.8)	0.715
20	117.4 (63.2, 171.5)	<0.001	143.5 (99.95, 187.1)	<0.001	26.2 (−29.8, 82.1)	0.359
24	136.5 (64.6, 208.4)	<0.001	170.1 (124.0, 216.1)	<0.001	33.5 (−39.1, 106.1)	0.365
40	N.A.**		276.2 (191.5, 360.8)	<0.001	N.A.**

^*P-value <0.05 was considered as statistical significance.

N.A.

^**Not analyzed: it is not feasible to fractionation proteome into greater than 24 fractions with current applied OFFGEL instruments.