Figure 5.
Effect of synaptic glutamate accumulation on ATP abundance. Incubation of cultured neurons with glutamate transporter inhibitor TBOA caused a marked reduction in ATP levels, which was blocked by receptor antagonist cocktail (AC). Co-application of TBOA and glutamate reduced ATP to a level similar to that by either glutamate or TBOA alone, suggesting a saturation by a single treatment, or a shared cellular process triggered by global and synaptic glutamate stimulation. *P<0.05, student’s t test.