Table 1.
3′-Peptidyl-tRNA-mimics obtained by NCL–desulfurization
| No | Sequence | Yield [%] | MWcalcd[amu] | MWobs[amu] |
|---|---|---|---|---|
| 1 | 5′-p-CUCCA-3′-NH-AFFRM | 65 | 2220.7 | 2220.6 |
| 2 | 5′-G3UGAU3CGAUCAC3ACCA-3′-NH-AL | 75 | 7160.5 | 7160.6 |
| 3 | 5′-G3UGAU3CGAUCAC3AC2A-3′-NH-AGFFM | 70 | 7529.9 | 7529.7 |
| 4 | 5′-G3UGAU3CGAUCAC3AC2A-3′-NH-AFFRM | 55 | 7629.1 | 7629.1 |
| 5 | 5′-G3UGAU3CGAUCAC3AC2A-3′-NH-ATLLM | 65 | 7506.0 | 7505.6 |
| 6 | 5′-G3UGAU3CGAUCAC3AC2A-3′-NH-ALVRM-NH2 | 65 | 7547.0 | 7546.6 |
| 7 | 5′-G3UGAU3CGAUCAC3AC2A-3′-NH-AWVRM | 70 | 7620.1 | 7619.9 |
| 8 | 5′-G3UGAU3CGAUCAC3AC2A-3′-NH-VGFFM | 20 | 7558.0 | 7557.6 |
| 9 | 5′-d(CTCCGGAACGCGCCTCC)dA-3′-NH-ALVRM | 65 | 5975.3 | 5975.1 |
| 10 | 5′-d(CTCCGGAACGCGCCTCC)dA-3′-NH-GALVRM | 55 | 6032.3 | 6032.6 |
RNA is annotated in the 5′ to 3′ direction; peptide from the C to N terminus.