Figure 1.

The effect of LPS dose on accumulation of nitrite in cell culture supernatant as assessed by the Griess assay (A; data are from three independent experiments (data expressed as mean±SEM)) and iNOS protein expression as assessed by Western blot analysis (B; representative blot from one of three independent experiments). LPS-induced iNOS protein expression was assessed over time (C; representative of three independent experiments) by Western blot analysis, (D) shows combined densitometry of iNOS expression from Western blot analysis (data are from three independent experiments and is expressed as mean±SEM) calculated using Quantity One software (BioRad).