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. 2012 Jun 15;366(2):404–419. doi: 10.1016/j.ydbio.2012.03.024

Fig. 7.

Fig. 7

Validation of CD41 as a direct target of Runx1 during early haematopoietic development. (A) Runx1 is recruited to the CD41 promoter during haematopoietic development. Raw ChIP-Seq read data were transformed into density plots and displayed as custom tracks within the UCSC genome browser above the UCSC track for gene structure (IgG control at the top followed by Runx1 data for populations 2–4). (B) The CD41 promoter can be activated by Runx1 and Runx1 together with Scl. The murine CD41 promoter region was inserted upstream of a luciferase reporter gene, and its activity monitored following co-transfection with Scl and Runx1 expression constructs in 293T cells. Relative luciferase activity values are normalised to empty vector control transfections. Values shown represent the average of 3 independent experiments, each performed in triplicate. (C) Early CD41 expression in mouse embryos critically depends on Runx1 activity. Immunostaining for CD41 in Runx1 heterozygous and Runx1 homozygous knock-in E7.5 embryos shows loss of CD41 expression in the absence of Runx1. (D) Scl, Gata2, Fli1 and Runx1 bind to the CD41 promoter in the murine haematopoietic progenitor cell line HPC7. ChIP-Seq results are displayed as density profile custom tracks within the UCSC genome browser as for A.