Figure 7.
CD4+CD45RO+CD25high+CD127−/loFoxP3+ T cells but not CD4+CD25−CD45RO− T cells inhibit KIM-1 expression by proximal tubulus epithelial cells (PTECs). A shows the gating strategy of the sorted T cell populations. B depicts IL-10 mRNA expression levels of activated PTECs, activated CD4+CD45RO+CD25high+CD127−/loFOXP3+ T regulatory (Treg) cells, and CD4+CD25−CD45RO− T (control) cells. C shows the results of the coculture experiments. T cells were activated by αCD3/αCD28 and IL-2 and were cocultured with activated PTECs for 3 days. To measure gene expression by the PTEC and T cell subsets, the T cells were separated from the PTEC layer by standard trypsinization procedures followed by Ficoll gradient centrifugation. KIM-1 expression by PTEC was inhibited by 63% (mean) when cocultured with Treg cells compared with control T cells.