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. 2012 Jul;92(4):328–332. doi: 10.1016/j.tube.2012.03.001

Figure 1.

Figure 1

WhiB1 binds at Rv0439c-Rv0440 (groEL2) intergenic region. (A) Radiolabeled Rv0439c-Rv0440 (groEL2) intergenic DNA was incubated with increasing concentrations of apo-WhiB1 before separation of protein-DNA complexes by electrophoresis. Lane 1, no protein; lanes 2–6 contain, 2.5, 5, 10, 20 and 40 μM apo-WhiB1, respectively. (B) The iron–sulfur form of WhiB1 (holo-WhiB1) does not bind at the Rv0439c-Rv0440 (groEL2) intergenic region. Lane 1, no protein; lane 2, apo-WhiB1 (20 μM); lane 3, holo-WhiB1 (20 μM). WhiB1 does not bind at the ahpC promoter. Lane 4, no protein; lane 5, apo-WhiB1 (20 μM); lane 6, holo-WhiB1 (20 μM). (C) Apo-WhiB1 binding to radiolabeled Rv0439c-Rv0440 (groEL2) DNA is inhibited by unlabeled Rv0439c-Rv0440 (groEL2) DNA but not by unlabeled rpfA promoter DNA. Lane 1, no protein; lanes 2–7 apo-WhiB1 (20 μM) in the absence (lanes 2 and 5), or presence of 100- and 50-fold molar excess unlabeled Rv0439c-Rv0440 (groEL2) DNA (lanes 3 and 6) or unlabeled rpfA promoter DNA (lanes 4 and 7). The locations of the free DNA (groEL2, ahpC) and the DNA-apo-WhiB1 complex (apo-WhiB1-groEL2) are indicated.