Figure 2.

Apo-WhiB1 inhibits transcription of groEL2 in vitro. Reactions in lanes 1, 3 and 5 contained: 0.1 pmole of template DNA (lane 1, template A; lane 3, template B; lane 5, template C, as shown in the upper panel), 1 pmole M. smegmatis RNA polymerase, 40 mM Tris–Cl pH 8.0, 10 mM MgCl₂, 70 mM NaCl, 1 mM EDTA, 1 mM DTT, 250 μg ml⁻¹ bovine serum albumin, 5% glycerol. Reactions in lanes 2 (template A), 4 (template B) and 6 (template C) were pre-incubated with apo-WhiB1 (20 μM) for 10 min at 37 °C. The sizes of the standard RNA molecules (lanes M) used to calibrate the gel are indicated.