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. 2012 Aug 30;3:317. doi: 10.3389/fmicb.2012.00317

Figure 6.

Figure 6

Mapping of the Manfredo mutL transcription to a promoter within the int pseudogene. Primer extension of the SpyCIM5 int cDNA revealed the presence of a cryptic promoter within the int pseudogene. The fluorescent primer (arrow labeled “F”) extended to an mRNA start position (35 nucleotides upstream) that exactly matched a predicted promoter (Reese, 2001) region (in capitals) and mRNA start (large open “C”) within the pseudogene ORF. The large arrow indicates the initiation and direction of the predicted mRNA. The encoded protein sequence of int is shown below for reference. No mRNA initiating from the region upstream of the integrase ORF could be detected. The X-axis of the figure corresponds to length of product in basepairs. The left Y-axis shows the relative fluorescence units of the sample peaks and right Y-axis shows the relative fluorescence units of the GeneScan 600 LIZ size standards. The small vertical arrow indicates the peak corresponding to the product of primer extension.