Figure 7.

Transcription from the SpyCIM5 int pseudogene is dependent upon host background. Gene chimeras were made that fused increasing portions of the SpyCIM5 int pseudogene and its upstream promoter to the green fluorescent protein gene (gfp); the constructs are shown below the micrographs. The downstream region of int following the 128 bp deletion is hatched in the figure. An encircled “P” indicates the predicted promoters (Reese, 2001). P1 is immediately upstream of the beginning of the int ORF, while P2 is positioned within the pseudogene and maps to the beginning of transcription (Figure 6). The plasmids with these constructs were introduced into strains NZ131 and Manfredo (SpyCIM5+). In E. coli JM109, no GFP fluorescence could be detected for any plasmid (not shown).