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. 2012 Jul 12;63(14):5189–5202. doi: 10.1093/jxb/ers179

Fig. 3.

Fig. 3.

Complementation of cbd and over-expression of Arabidopsis cpSRP54 in WT Arabidopsis. (A) Representative phenotypes of Arabidopsis cbd lines complemented with an Arabidopsis cpSRP54 gDNA or CDS. Three-week-old seedlings are shown. From left to right: non-transformed cbd, cbd transformed with empty vector pMDC83, cbd with pMDC83-cpSRP54 CDS, cbd with empty vector pMDC99, and cbd with pMDC99-cpSRP54 gDNA. (B) Representative carotenoid content of 8-day-old seedlings. Value are mean ± standard error of three cbd-complemented lines per construct and three over-expression lines per construct. Significant difference was set at P ≤ 0.05 (*) and P ≤ 0.01 (**) relative to WT Arabidopsis. (C) Semi-quantitative RT-PCR confirmation of cpSRP54 expression in cbd-complemented and over-expression transgenic lines as indicated. The CDS of cpSRP54 was amplified and the AtACT2 gene was used as an internal control. NT, non-transformed control.