Table 1.
Primers used to characterize Arabidopsis cbd mutant
| Primers used for genotyping and molecular complementation | ||||
| Primer | AGI code | Sequence (from 5′ to 3′) | Purpose | |
| pSKTAIL-L1 | N/A | TTCTCATCTAAGCCCCCATTTGG | Confirmation of T-DNA insertion site in cbd mutant | |
| LBb1 | N/A | GCGTGGACCGCTTGCTGCA | Confirmation of T-DNA insertion site in SALK lines | |
| P1 | AT5G03940 | ACCGGTTCTTGTTTTTGTTTCATTAGCA | Confirmation of T-DNA insertion site in CS850421 | |
| P2 | AT5G03940 | CATTCGCTCTCCACGTCCTACCAGTTTA | Confirmation of T-DNA insertion site in CS850421 | |
| P3 | AT5G03940 | AGCCCCATTGTCAGGTTATTTCGCA | Confirmation of T-DNA insertion site in SALK_079668 | |
| P4 | AT5G03940 | GAGCTCACGGGCAACGAAATAAGAAAGA | Confirmation of T-DNA insertion site in SALK_079668 | |
| P5 | AT5G03940 | TATTATGGATACTGCAGGGAGGCTTCA | Confirmation of T-DNA insertion site in cbd | |
| P6 | AT5G03940 | TTGTTCTGCTTTCAATGCGTCCTCG | Confirmation of T-DNA insertion site in cbd | |
| P7 | AT5G03940 | ATGGAGGCTCTTCAATTTTCCAGCGT | Forward primer for amplification of cpSRP54 coding region | |
| P8 | AT5G03940 | GTTACCAGAGCCGAAGCCACGAGGA | Reverse primer for amplification of cpSRP54 coding region | |
| P9 | AT5G03940 | GTTTTCCTTTGGTTCAAATTTCCATTTA | Forward primer for amplification of cpSRP54 genomic sequence | |
| P10 | AT5G03940 | GTATTTTAGTTATGCAAAGCCTATGAA | Reverse primer for amplification of cpSRP54 genomic sequence | |
| AtACT2-F | AT3G18780 | GATATGGAAAAGATCTGGCATCAC | Forward primer for amplification of AtACT2 | |
| AtACT2-R | AT3G18780 | TCATACTCGGCCTTGGAGATCCAC | Reverse primer for amplification of AtACT2 | |
| Gene-specific primers used in quantitative real-time PCR | ||||
| Gene | AGI code | Forward primer (from 5′ to 3′) | Reverse primer (from 5′ to 3′) | Reference |
| PSY | AT5G17230 | TGCGGTGAAGTTTGCGCTGA | TGAAGCATTTGGCCCATCCA | |
| PDS | AT4G14210 | GTCGGTCACGCGCTCAGGTA | CGAGATGCTGACATGGCCAGA | |
| ZDS | AT3G04870 | CCATCGTCACGAGGCCTAGAA | TGTGTATGAACCGGCGAGGA | |
| bLYC | AT3G10230 | TGGTAGCGCTGCTCTTTTGGA | ACCAGCAGGACCACCACCAA | |
| BCH1 | AT4G25700 | GGCACGCTTCTCTATGGAATATGCATGA | GAATCCATAAGAGAGGAGACCAATCGCT | |
| BCH2 | AT5G52570 | ATGGAGTTTTGGGCAAGATGGGCTCAT | CAGGAACCGCGTTTGTTATAGCAAACACA | |
| LUT1 | AT3G53130 | CGAAATCCCAATCATGGGTCA | GCACCTCCGAGGAGATCAGC | |
| ZEP | AT5G67030 | ATGACCGGCTTCGAGAGTGG | TTCCGACGATGCAAGGTTGA | |
| VDE | AT1G08550 | ACCGCTCCGCTGTTGCTAAA | TGGCAATGCACTTTGCGAGT | |
| CCD1 | AT3G63520 | TGTTCCGCGTGAGACAGCAG | CCACCACTGCCACCGGTTC | |
| CCD4 | AT4G19170 | AAGATCTCCGGTGTGGTGAAGC | CCGGATTACCAGGATCCCTAGC | |
| CCD7 | AT2G44990 | CTAAACCGTGGCGACGACAA | CCGGAAAATCTGACGGCTTG | |
| CCD8 | AT4G32810 | CGTTTATGCATGCGGTGCTC | GGTCGAGGCACGAAGAATGG | |
| CAO | AT1G44446 | CCGGTGGAACACGGTTTACTTCTAGATA | AGTATCCTTGGAGACCCGAGGTAGGTGT | |
| PORA | At1g03630 | CTCGGTGTTTCAACCTTTGG | CAGATAGAATCAGCCAAAACACAAC | Kakizaki et al. (2009) |
| PORB | AT4G27440 | TTCCGAGAGCACATTCCTCTCTTCCGT | CCCTGATTTCGTCAAGCTTGGATCACT | |
| Lhca2 | AT3G61470 | ACATCTACACTGGCACTGGTCCTATTGA | ACACACAAACGCATTCACCTCCCCATAA | |
| Lhcb1 | AT1g29930 | AGCTCAAGAACGGAAGATTGG | GCCAAATGGTCAGCAAGGTT | Kakizaki et al. (2009) |
| Lhcb2.1 | AT2g05100 | AAGTCGTGAATGTACTTATTGGTG | GGTGGTGTGGTTCATTAAAGGT | Kakizaki et al. (2009) |
| LSU | ATCg00490 | TTGCCGAGATAATGGCCTACTT | AACAAAGCCCAAAGTTGACTCC | Kakizaki et al. (2009) |
| OE33 | AT5G66570 | ACCGTCAAGGCAGACAGTGTAAGCAAGA | CTTGAAATTGACGCTTCCGTCTGAAGCA | |
| PSBA | ATCG00020 | GTGTATTCGGCGGCTCCCTTTTTAGT | CTTCTTCTTGCCCGAATCTGTAACCTTCA | |
| PSBS | AT1G44575 | CATTGGAGCTCTCGGAGACAGAGGAA | CTCGTTCGCCTTCGTGAACCCAAACAAT | |
| NCED3 | AT3g14440 | TAACGCCGTTAGCTTAGAGGTTGAAGCA | AGAACTCACACGACCTGCTTCGCCAAAT | |
| NCED5 | AT1G30100 | TGTTCACGACGAGGAGAGTTGG | TATCCGCCGAATTCACGAAAGT | |
| NCED6 | AT3G24220 | GAGCTGGGATCGGTCTAGTGGA | TTGACCGTCGATCTTCACTTGG | |
| NCED9 | AT1G78390 | TCGACGGAGACGGTATGGTACA | TCTCCAATTGCTTTGGGGAAAA | |
| ABA2 | AT1G52340 | ACGGTTGATGATGTAGCGAACGCTGTT | CATCTGAAGACTTTAAAGGAGTGGTTAG | |
| AAO3 | AT2G27150 | TGGACTGCTCCTTCTGGTGATG | CTTGATGCTTCTTGGCGAGACA | |
| CYP707A1 | AT4G19230 | ATTTGATCCATCAAGATTCGAGGTGGCT | ACCAAACCTGTACTTGGTGGTGAGATGA | |
| CYP707A2 | AT2G29090 | GCGAATCCATCACTCCTCCGAATTCTT | TCACTTCCTGGACATGAGTGCACTCCAT | |
| CYP707A3 | AT5G45340 | CGAGATTCGAAGTTGCGCCGAAACCGA | GATTGACCATCTGTACTTAGTGGTGAGA | |
| CYP707A4 | AT3G19270 | GTGTGCTAACCCAAGAACAGATTGCAGA | CAGCCTTAACAGCTTCTAGAAGTTTCTGA | |
| At5g12240 | At5g12240 | TGGCACGATGCACCGACTGTTGCT | CTAGTCAATCTAACA AGCCAGTAAGCTA | Czechowski et al. (2005) |
| UBC | At5g25760 | TGCTTGGAGTCCTGCTTGGA | TGTGCCATTGAATTGAACCCTCT | Czechowski et al. (2005) |