Table 2.

Primers used in this study to generate and characterize transgenic cpSRP54 ⁺ B. napus lines

Primers used for vector construction and confirmation of transgenic lines
Primer name	Sequence (from 5′ to 3′)	Purpose
FFC-F31	ggatccGTACCAAGAA GATTGCGGAG	Cloning of cpSRP54 (FFC) genomic sequence, add BamHI site (in lower case)
FFC-R34	gtcgacGTATTTTAGTTATGCAAAGCCTATGAA	Cloning of cpSRP54 (FFC) genomic sequence, add SalI site (in lower case)
napin-F5	AGCTCCCAATTTATATTCCCAACGGCAC	Confirmation of transgenic lines
FFC-R6	GAGCTCACGGGCAACGAAATAAGAAAGA	Confirmation of transgenic lines
Gene-specific primers used in quantitative real-time PCR
Gene name	Forward primer (from 5′ to 3′)	Reverse primer (from 5′ to 3′)	Reference
cpSRP54	ACAAAAGGCTCCACCTGGAACTGC	AGCCGAAGCCACGAGGACCA	Fujisawa et al. (2009)
BnbLYC	TCCACTGTTGTCTGCAGTGACG	CATCGACCTCAGCAACGATACC	Fujisawa et al. (2009)
BneLYC	TGAGGAGGTGTGTGGAGTCAGG	GAAGCTGCTCCAGAAGCAACAG	Fujisawa et al. (2009)
BnBCH	CAGAGGCTTCTCGGTCTGCTAC	CCTCTCGGACTTCTTCCTCTCC	Fujisawa et al. (2009)
BnCRTISO	GAGGTGGCAGCTGGAATCATAC	TCCTCTTGGCATTGGTCCATAC	Fujisawa et al. (2009)
BnPDS	GGCTGCAGTGGAAGGAACACTC	TCTCTGGCCATGTCAGCATCTC	Fujisawa et al. (2009)
BnPSY	CCAAAGCAACGACCGAGAGTG	CATCTGAGAGACCAGCCTGAGC	Fujisawa et al. (2009)
BnVDE	TCACGACCGTACGAGATTCTTC	AATCCAGATAAGGGTCGTGAGG	Fujisawa et al. (2009)
BnZDS	GCAATGAAAGACATTCGCAACC	TCTCGCACTCATGTTGTCACAG	Fujisawa et al. (2009)
BnZEP	TGCTGAAGAAGTCATGGAAGCTG	CTGCTAATCACCCGAGTCACAGG	Fujisawa et al. (2009)
BnACT3	GCATCCCTCAGCACTTTCCAACAGA	ACCACGAACCAGAAGGCAGAAACACT