Skip to main content
. 2012 Aug 29;63(14):5323–5335. doi: 10.1093/jxb/ers190

Fig. 4.

Fig. 4.

Genotype and fertility of BC2F2 and BC3F2 populations segregated for T-DNA inserted in the RAD51C gene. 39630-m-F and 39630-m-R are RAD51C-specific PCR primers, and RB1 is a T-DNA-specific primer. Plants showing only the band amplified by 39630-m-F and RB1 were RAD51C-KO plants with a homozygous T-DNA insertion. Plants showing only the band amplified by 39630-m-F and 39630-m-R were negative plants without a T-DNA insertion. Plants showing both PCR amplification bands were heterozygous plants with heterozygous T-DNA insertion. The bar represents the mean (three panicles) ±SD. (A) Plant fertility of a BC2F2 population developed from the cross between the RAD51C-T-DNA insertion line (4D-50016) and wild-type (WT) Dongjin. (B) Plant fertility of a BC3F2 population developed from the cross between the RAD51C-T-DNA insertion line and rice variety Zhonghua 11 (ZH11).