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. 2012 Aug 29;63(14):5351–5364. doi: 10.1093/jxb/ers196

Fig. 7.

Fig. 7.

Q0990>>C4 leaf venation patterning. Darkfield (A–C, E–H) or Nomarski (D) images of 2-week-old wild-type (A, E), Q0990>>C4.1 (B, C, F, G), or Q0990>>C4.2 (D, H) leaves grown on control media (A–D) or media supplemented with 10 µM N-naphthylphthalamic acid (NPA; E–H). Q0990>>C4.1 leaves had distorted secondary venation patterning and discontinuous secondary and higher-order veins (B, arrow in C), while Q0990>>C4.2 leaves often lacked secondary vein formation (D; arrows delimit midvein). NPA treatment induced vascular overgrowth in wild-type (E) and Q0990>>C4 (F–H) leaves, although vein discontinuities were still evident in some Q0990>>C4.1 leaves (e.g. arrow in G) and in Q0990>>C4.2 (H, veins barely extending from midvein region). Q, Q0990. Bars, 500 µm (A–C, E–G), 100 µm (D, H).