Table 1. Phylum(and genus)-level assignments of operational taxonomic units (OTUs) in the scent pouches of female hyenas.
| Phylum | Number of OTUs | Percentage of Sequences (± SD) | Genera |
|---|---|---|---|
| Firmicutes | 236 (55) | 73.3 ± 13.9 (22.9 ± 14.6) | Anaerococcus, Anaerovorax, Bacillus, Blautia, Clostridium, Coprobacillus, Dialister, Eubacterium, Facklamia, Finegoldia, Fusibacter, Helcococcus, Holdemania, Ignavigranum, Macrococcus, Peptococcus, Peptoniphilus, Peptostreptococcus, Sedimentibacter, Sporacetigenium, Staphylococcus, Streptococcus, Tissierella |
| Actinobacteria | 53 (34) | 17.1 ± 14.0 (17.0 ± 14.0) | Actinomyces, Arthrobacter, Blastococcus, Brachybacterium, Cellulomonas, Corynebacterium, Curtobacterium, Dietzia, Friedmanniella, Janibacter, Kineosporia, Marmoricola, Micromonospora, Olsenella, Phycicoccus, Propionibacterium, Rubrobacter, Virgisporangium |
| Bacteroidetes | 27 (14) | 3.1 ± 3.2 (3.1 ± 3.1) | Bacteroides, Flavisolibacter, Paludibacter, Parabacteroides, Paraprevotella, Pedobacter, Petrimonas, Porphyromonas, Prevotella |
| Proteobacteria | 27 (23) | 1.1 ± 3.1 (1.0 ± 3.1) | Acinetobacter, Aeromonas, Anaeromyxobacter, Arcobacter, Bradyrhizobium, Comamonas, Enhydrobacter, Herbaspirillum, Massilia, Neisseria, Novosphingobium, Pantoea, Phenylobacterium, Pseudomonas, Sphingomonas, Stenotrophomonas, Sutterella |
| Chloroflexi | 13 (3) | 0.1 ± 0.1 (< 0.1 ± 0.0) | Bellilinea, Longilinea, Sphaerobacter |
| Fusobacteria | 4 (3) | 0.1 ± 0.3 (0.1 ± 0.3) | Cetobacterium, Fusobacterium |
| Acidobacteria | 6 (6) | < 0.1 ± 0.0 (< 0.1 ± 0.0) | Gp4, Gp6, Gp7, Gp17 |
| Tenericutes | 1 (1) | < 0.1 ± 0.0 (< 0.1 ± 0.0) | Anaeroplasma |
| Gemmatimonadetes | 1 (1) | < 0.1 ± 0.0 (< 0.1 ± 0.0) | Gemmatimonas |
| Classified Totals | 368 (140) | 94.8 ± 4.1 (44.1 ± 24.5) | 78 Characterized Genera |
Taxonomic assignments of OTUs were determined using the Ribosomal Database Project's Classifier tool with an 80% confidence threshold51. Most of the sequences that remained unclassified at the phylum level (4.9/5.2%) belonged to OTU 21 (see Figure 3), which fell just short of being placed in the phylum Firmicutes with a confidence of 79%.