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CREB phosphorylation is decreased by ANG II in mice lacking PKC-α. A and B: total CREB abundance was assayed in the whole IM of WT (A) or PKC-α−/− (B) animals by Western blot analysis with an anti-CREB antibody and remained unchanged by ANG II in both groups. CREB phosphorylation was assayed by anti-phospho-CREB antibody. Top, representative Western blots; bottom, average densitometries. n = 12 animals/group. *P < 0.05.
