Fig. 7.

Subcellular distribution of NCC, ROMK, and AQP2 determined by differential centrifugation of 2K1Na and 0K1Na homogenates (n = 3 each) at 17,000 g to pellet-enriched plasma membranes (PM) and centrifugation of the 17,000 g supernatants at 150,000 g to pellet-enriched intracellular membranes (ICM) (43). Equal protein amounts of PM and ICM, as indicated, were analyzed by immunoblot, and the results illustrate significant differences in the PM-to-ICM ratio. Arrows indicate specific bands of interest.