Fig. 5.

drice knockdown inhibits PE-mediated cell death. (A) S2 cells were treated with dsRNA to drice followed by western blot analysis to detect endogenous Drice. dsRNA to a non-specific target gene, gfp, was used as control. Anti-tubulin was used as a loading control. The asterisk denotes a non-specific background band. S2 cells were subjected to RNAi using dsRNA to drice and treated with 150ng/ml actinomycin D (B), 200μg/ml cycloheximide (C) or 100ng/ml PE (D) for 48 h. Cell viability was measured using alamarBlue® ® and the data is presented as percentage value of the untreated sample. Each bar is the mean of at least three determinations. Error bars represent one standard deviation from the mean.