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. Author manuscript; available in PMC: 2013 Mar 23.
Published in final edited form as: Immunity. 2012 Mar 15;36(3):348–361. doi: 10.1016/j.immuni.2012.02.010

Figure 5. CCR7 and IL7Rα fail to be efficiently up-regulated inId2 f/fId3f/fCd4cre+CD4 SP thymocytes.

Figure 5

(A) Representative staining of thymus from indicated genotypes. Plots are pre-gated on DP (CD4+CD8+) or CD4 SP (CD4+CD8−TCRβ+) thymocytes and percentages in each quadrant are displayed. Percent means ± SD for total CCR7+ thymocytes within the CD4 SP compartment were 78 ± 8.0 for cre− (n=6) and 25 ± 12 for cre+ (n=6), with a significant difference (p<0.001). Percent means ± SD for total CXCR4− thymocytes within the CD4 SP compartment were 86 ± 4.6 for cre− (n=4) and 80 ± 12 for cre+ (n=4). (B) Representative staining of thymus with histograms pre-gated on DP and CD4 SP thymocytes as described in (A). Percentages within the IL7Rα+ gate are displayed. Percent means ± SD for IL7Rα+ thymocytes within the CD4 SP compartment were 55 ± 4.1 for cre− (n=3) and 3.7 ± 1.6 for cre+ (n=3), with a significant difference (p<0.001). (C) Quantitative RT-PCR analysis of Klf2 and Tox expression in sorted DP (CD4+CD8+) and CD4 SP (CD4+CD8−TCRβ+) thymocytes. Duplicate runs from a sample sorted from an individual animal (n=3 for cre− DP, cre+ DP, cre− CD4 SP; n=4 for cre+ CD4 SP) were averaged and normalized to the expression of Gapdh. Graphed results are means with error bars representing SEM. (D) Representative staining of thymus with histograms pre-gated on DP and CD4 SP thymocytes as described in (A). Percentages within the HSAlo gate are displayed (HSA clone M1/69 was used). Percent means ± SD for HSAlo thymocytes within the CD4 SP compartment were 53 ± 6.0 for cre− (n=4) and 86 ± 8.4 for cre+ (n=4), with a significant difference (p<0.001) Plots in (A, B, and D) are from analysis of the same individuals. See also Figure S5.