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Effects of HSP90 inhibition on EGFR stability. To assess whether inhibition of HSP90 affects stability of EGFR, UMSCC1 cells were treated with AT13387 (30 nM) or DMSO for 12 hours, followed by CHX (100 µg/ml) to block the new protein synthesis. (A) The level of EGFR was assessed at multiple time points using immunoblot analysis, and half-life was calculated. Inhibition of HSP90 reduced the half-life of EGFR from 8 to 3.7 hours. (B) Representative blots of EGFR are shown.
