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. 2012 May 1;303(2):E191–E199. doi: 10.1152/ajpendo.00465.2011

Fig. 2.

Fig. 2.

Effect of NADK overexpression on NADK mRNA (A), total (NADPH + NADP+) levels (B), NADPH/(NADPH + NADP+) ratio (C), insulin secretion (D), and NADH/(NADH + NAD+) and ATP/ADP ratio (E) in INS-1 832/13 cells. Nucleotides were determined using NADPH/NADP+, NADH/NAD+, and ATP/ADP kits (Abcam, Cambridge, MA) according to the manufacturer's protocol. Insulin secretion was determined by ELISA (Alpco, Salem, NH). Data are means ± SE from 3–5 independent experiments performed in duplicate or triplicate measurements. 2G, 2 mM glucose; 16G, 16 mM glucose. *P < 0.05 NADK(+) vs. NADK control; #P < 0.05 2G vs. 16G.