(a) Whole cell lysates containing corresponding GALT proteins (wild-type and variants) from same amount of cell culture were separated by SDS-PAGE and detected by anti-His6 tag antibody. * Wild-type, non-induced. ** Wild-type, induced. M, molecular weight marker.
(b) GALT proteins (wild-type and variants) purified from same amount of cell culture were separated and detected as in (a).