Table 1.
Enzymatic parameters of six active variants
KM gal-1-p (mM) | KM UDP-Glu (mM) | Vmax (nmol/mg/sec) | |
---|---|---|---|
WT | 1.25±0.36 | 0.43±0.09 | 804±65 |
R201C | 1.89±0.62 | 0.58±0.13 | 396±59 |
E220K | 2.34±0.42 | 0.69±0.16 | 253±53 |
R223S | 1.12±0.31 | 0.76±0.09 | 297±25 |
I278N | 1.98±0.35 | 1.23±0.28 | 45±3 |
L289F | 2.14±0.21 | 0.48±0.13 | 306±23 |
E291V | 2.68±0.16 | 0.95±0.43 | 385±18 |
Mean values and standard deviations presented here were calculated from three separate experiments.