Skip to main content
. Author manuscript; available in PMC: 2012 Nov 1.
Published in final edited form as: J Autoimmun. 2011 Jul 13;37(3):217–227. doi: 10.1016/j.jaut.2011.06.002

Fig. 3. aTreg cells are dependent on IL-7 in vivo.

Fig. 3

(A) Polyclonal aTreg cells were differentiated from CD4+CD25−GFP− cells from NOD.FoxP3-GFP reporter mice. The cells were injected into NOD.Scid recipients and treated with control mouse IgG or anti-IL-7 neutralizing antibody at the time of cell transfer and on days 3 and 7, in a dose of 300μg/injection. Ten days after cell transfer, the cells from the spleen were analyzed for the presence of GFP+ cells (n = 5/group, mean ± SEM). (B) aTreg cells were differentiated from CD4+CD25− cells from B6.CD45.1 mice. The cells were transferred into IL-7−/− or IL-7R−/− recipients in a dose of 3 × 106/recipient. The recipient mice were evaluated on day 14 after cell transfer. The recovery of FoxP3+ donor cells (left) was calculated, and IL-7Rα expression (right) was analyzed by flow cytometry after gating on FoxP3+ donor cells from the spleen (n = 5/group).