Table 2.
Effect of specific nucleofection programs on cell survival and transfection efficiency of human primary calvarial suture mesenchymal cells
| |
|
V- Nucleofector buffer |
L- Nucleofector buffer |
||
|---|---|---|---|---|---|
| Nucleofection program | pmaxGFP (μg) | % Cell survival | % Transfection efficiency | % Cell survival | % Transfection efficiency |
| No-Program control |
3μg |
99.2 |
0.2 |
98.8±0.7* |
0.4±0.1* |
| T-030 |
No GFP control |
26 |
0.3 |
37.0 ±7.0* |
0.6±0.2* |
| T-030 |
3μg |
14 |
35.5 |
19.0±2.0* |
56.2±9.1* |
| A-020 |
3 μg |
20 |
10.4 |
24 |
3.8 |
| T-020 |
3 μg |
14 |
22.7 |
17 |
31.6 |
| X-001 |
3 μg |
24 |
12.8 |
27 |
8.0 |
| X-005 |
3 μg |
24 |
16.5 |
20 |
13.7 |
| L-029 |
3 μg |
11 |
23.5 |
18 |
22.3 |
| D-023 | 3 μg | 15 | 27.2 | 20 | 14.8 |
Values ± SD with * represent the mean of 2 independent experiments, each with 2 from biological replicates. The other values are derived from one experiment with 2 biological replicates.