Table 4.
Effect of increasing cells on cell survival and transfection efficiency using the cell line L kit and T030 program
| Program | Cells | pmaxGFP (μg) | % Cell survival | % Transfection efficiency | % Cell input, alive and transfected |
|---|---|---|---|---|---|
| T-030 |
500,000 |
No GFP control |
36.0 ±4.2* |
0.4 ±0.2* |
0.1 |
| T-030 |
500,000 |
3 μg |
19.0 ±0.8* |
61.3 ±5.5* |
11.6 |
| T-030 |
1,000,000 |
3 μg |
13.0 ±1.0# |
88.5 ±2.3# |
11.5 |
| T-030 | 1,500,000 | 3 μg | 11.0 ±1.2# | 89.4 ±2.0# | 9.8 |
Values ± SD with * represent the mean of 4 independent experiments, each with 4 biological replicates and values with # represent the mean of 2 independent experiments, each with 2 biological replicates.