Figure 2.
Levels of transcription of theTcrHTgene in BZ resistant and susceptible T. cruzipopulations and strains showing: (A) Northern blot profiles of total RNA extracts obtained using a 32P-labeled TcrHT-specific probe; (B) the quantitative control in which the same membrane was exposed to a T. cruzi 24 S ribosomal RNA probe; and (C) levels of TcrHT mRNA as determined quantitatively (relative to the single-copy housekeeping gene TcHGPRT) by real-time RT-PCR. Mean values of the copy number ratio TcrHT/TcHGPRT ± standard deviations from three independent experiments are indicated. The mean values for 17 WTS and 17 LER are significantly different (* p <0.001), whilst the BZR versus BZS and the CL versus Colombiana mean values show no difference.