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. 2012 Aug 4;5:409. doi: 10.1186/1756-0500-5-409

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Copyright ©2012 Abirached-Darmency et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Light microscopy immuno-detection : the anti-vicilin antibodies are visualised with Alexa 488 green fluorescenceand counterstained with the red fluorescent propidium iodide. (A) Embryo cells 16 DAP showed green fluorescent vicilin bodies (vib) and the initiation of vacuole fragmentation. (B) Note an increase in the number of vicilin bodies and the presence of a multiple vacuolar compartment (*) at 20 DAP. vb, Vascular Bundle; vc, vacuolar compartment. The scale bar corresponds to 4 μm.