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. 2012 Aug 30;8(8):e1002922. doi: 10.1371/journal.pgen.1002922

Figure 4. Ccdc94 interacts with core members of the Prp19 complex.

Figure 4

(A) TAP/MS analysis shows that human CCDC94 binds to core members of the PRP19 complex. “Total Peptide #” indicates the number of peptides present in the analysis that are derived from the indicated protein. “Coverage Percentage” refers to the percent of protein sequence for a given protein that is represented by the peptides. “Unique # Peptide” refers to the number of unique peptides found for the indicated protein. (B, C) Wild-type embryos were injected with the ccdc94 atg morpholino (400 µM), prp19 e3i3 morpholino (200 µM), or mismatch morpholino (as a negative control and to keep the total concentration of morpholino injections at 600 µM) and grown to 22 hpf. A range of phenotypes were observed in the embryos as shown in (B). Embryos showing the different phenotypes from (B) were quantified in (C). (D) Wild-type embryos were injected with the ccdc94 atg morpholino (400 µM), plrg1 atg morpholino (20 µM), or mismatch morpholino (as a negative control and to keep the total concentration of morpholino injections at 420 µM), grown to 22 hpf, and analyzed as in (C). Data in (C) and (D) is derived from one representative experiment; however, three independent experiments were qualitatively similar for each panel. Number (n) of embryos analyzed for each group is indicated. See also Figure S7.