Figure 3. Temporal expression profiling for individual proteins identified in synapse-enriched fractions undergoing degeneration.

Temporal profiles of protein expression changes in degenerating synapse-enriched fractions were grouped into 3 distinct categories: proteins with expression changes >20% by 24 hours, with further progressive alterations by 48 hours (A); proteins up or down regulated >20% by 24 hours following injury but with no subsequent increase/decrease (B); and proteins not changed at 24 hours but up or down regulated >20% at 48 hours following injury (C). Proteins responding within 24 hrs of lesion were considered to represent immediate-early responders and initiators of the degeneration process, whereas proteins whose expression levels were found to be altered only at 48 hrs after the lesion were considered to represent effector pathways involved with the onset of degeneration.