Figure 6. Detailed genetic analysis confirms DNAJC5/CSP as a robust regulator of axo-synaptic degeneration in vivo.

Representative confocal micrographs showing axon degeneration profiles in wild-type (WT) flies and additional DNAJC5/CSP lines: csp^X1, a loss of function allele which deletes the first exon of csp; and Df(3R)Exel6138, a deletion which completely removes the csp locus. Examples are shown of uninjured axons (left panels), unilaterally injured axons (middle panels) and bilaterally injured axons (right panels). Both csp^X1 and Df(3R)Exel6138 failed to complement the delay in axonal degeneration observed with our original allele (csp^DG29203), thereby mapping this phenotype to the csp locus. Note how the severity of the delay in axonal degeneration was enhanced when csp^DG29203 was placed over either of these null alleles of csp, suggesting that csp^DG29203 is a weak loss of function allele.