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. 2012 Aug 30;8(8):e1002879. doi: 10.1371/journal.ppat.1002879

Figure 3. Fas2Δ/Δ yeast cells did not arrest cell cycle progression upon serum incubation.

Figure 3

A, The survival of Fas2Δ/Δ yeast cells in serum was enhanced by 75 mM hydroxyurea (HU). The yeast cells before (0 h) and after (24 h) serum incubation were serially diluted and spotted on YPD media for WT and YPDT40 for Fas2Δ/Δ strain. B, DNA content of the WT and Fas2Δ/Δ strains before (0 h) and after 1–4 h of serum incubation with the presence or absence of 75 mM hydroxyurea was measured by FACS. Cultures were collected at indicated intervals and processed for DNA content analysis by staining with Sytoxgreen. The percentages of yeast cells at G1 and G2 stage were calculated. C, Cycloheximide (CHX) rescued C. parapsilosis FAS2 mutant cells from serum induced death. In contrast, inhibition of protein synthesis by CHX slightly reduced WT and reconstituted (Fas2Δ/FAS2) C. parapsilosis yeast cell growth. Experiments were performed twice with similar results.