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. 2012 Aug 30;8(8):e1002879. doi: 10.1371/journal.ppat.1002879

Figure 6. Cell death is dependent on mtDNA.

Figure 6

A, mitochondrial DNA and genomic DNA were evaluated by PCR from total DNA isolated from the indicated strains. Mitochondrial DNA content was assessed using the subunit I of cytochrome c oxidase gene (Cox1). Genomic DNA content was determined using the actin gene (Actin). B, Deletion of mtDNA protected Fas2Δ/Δ yeast cells from death induced by culture in serum. WT, Fas2Δ/Δ and Fas2Δ/Δ with partially deleted mtDNA (Fas2-rho−) were incubated with 50% serum for 24 and 48 h. Aliquots were serially diluted and WT yeast were spotted onto YPD whereas the Fas2Δ/Δ mutants were inoculated onto YPDT40. Experiments were performed twice with similar results. C, A proposed model of yeast cell sensitivity to glucose in the media in the setting of fatty acid inhibition.