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. 2012 Aug 30;8(8):e1002900. doi: 10.1371/journal.ppat.1002900

Figure 3. Genetic diversity of VSG427-3 (224) switchers.

Figure 3

(A) Comparative maps of BES1 (active) and BES7 (silent; source of VSG427-3) with critical experimental features illustrated: HindIII sites, genes (ESAG1 & VSG Ψ), and BES7-unique probe (red line). (B) PCR products from 18 VSG427-3 switcher originating from 3 VSG427-2 starting clones (#2, #3, & #5) are shown for BES1 VSG Ψ, BES1 ESAG1, and Tubulin control in comparison to positive (WT) and negative (BES1 deletion strain courtesy of Kim & Cross). The control strain demonstrates the presence or absence of features in the genome. (C) FIGE Southern blot analysis of VSG427-3 switchers from HindIII digested gDNA probed with BES7-unique probe (BES7 red underline). Illustrations and predicted sizes of restriction fragments shown to the right of the blot image.