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. 2012 Aug 30;7(8):e44472. doi: 10.1371/journal.pone.0044472

Figure 5. Effects of UV and FICZ on the EGFR signalling in NHEK.

Figure 5

A. Western blot detection of the phosphorylated forms of EGFR (P-EGFR) and ERK (P-ERK) in total lysates of untreated NHEK (-) and post-UV irradiation; B. Densitometry of the bands. *P<0.05 and §P<0.01 versus untreated controls (-). C. Western blots of nuclear non-phosphorylated (nEGFR) and phosphorylated EGFR (nP-EGFR) before and 20 min after exposure to UV. Histone 4 (H4) was used as a loading control; D. Densitometry of nEGFR and nP-EGFR bands. *P<0.05 versus untreated control (-). E. Western blot detection of the phosphorylated forms of EGFR (P-EGFR) and ERK (P-ERK) in total lysates of untreated NHEK (-) and after 30 min exposure to 0.1 or 1.0 µM FICZ. F. Densitometry of the bands. *P<0.05 versus untreated control (-). G. Western blots of nuclear non-phosphorylated (nEGFR) and phosphorylated EGFR (nP-EGFR) after 30 min exposure to 0.1 or 1.0 µM FICZ; H. Densitometry of nEGFR and nP-EGFR bands. *P<0.05 versus untreated controls.