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. 2012 Aug 30;7(8):e43501. doi: 10.1371/journal.pone.0043501

Figure 1. Molecular characterization of wild type and transgenic maize plants overexpressing TsVP.

Figure 1

(A) polymerase chain reaction (PCR) analysis of wild type and transgenic lines with specific primers for TsVP. Lane M, DNA marker DL2000. Lane +, PCR result of plasmid pCAMBIA1300-Ubi-TsVP-als. Lane WT, wild type. Lanes L1 to L5, transgenic lines. (B) analysis of the expression of TsVP in wild type and transgenic lines by reverse transcription - polymerase chain reaction (RT-PCR) using maize actin1 as an internal control. Lane (−), H2O. Lane WT, wild type. Lanes L1 to L5, transgenic lines. (C) southern blot analysis of wild type and transgenic lines. Maize genomic DNA (50 µg) was digested with restriction enzyme BamHI that contained no cut site in the TsVP cDNA region. A PCR - amplified fragment containing full - length TsVP cDNA was used as a probe. Lane M, λDNA/EcoT14I molecular weights. Lane WT, wild type. Lanes L1 to L3, transgenic lines.