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. 2012 Aug 30;7(8):e43752. doi: 10.1371/journal.pone.0043752

Figure 4. JAM-A expression and migration abilities of breast cancer cells.

Figure 4

A: Mean Fluorescence Intensities (MFI) of CD146 and JAM-A expression on indicated human breast cancer cell lines are shown. Values indicate the specific mean fluorescence intensity (sMFI) ± the standard error of the mean of at least seven experiments. The sMFI was defined as the ratio of the MFI for the considered antibody over the MFI obtained with the appropriate isotypic control. B: down-regulation of JAM-A expression by over-expression of CD146 in MCF-7 cells. JAM-A expression measured by flow cytometry in CD146+ MCF-7 cells, one representative experiment. C, migration of mock-transfected or CD146+ MCF-7 cells after JAM-A knockdown. JAM-A was inhibited with a siRNA in mock transfected and CD146+ MCF-7 cells. Results represent the mean ± standard error of the mean of three independent experiments.