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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Nat Neurosci. 2012 Jul 22;15(9):1227–1235. doi: 10.1038/nn.3178

Figure 1. Localization of Prion Protein to the Post-Synaptic Density.

Figure 1

a The indicated subcellular fractions (20 µg protein) were analyzed by immunoblot with anti-PrPC, anti-Fyn, anti-PSD95, anti-synaptophysin, and anti-actin antibodies. The lower two panels are from a separate preparation.

b Quantification of PSD-95, PrPC or Fyn levels normalized to actin. Data are mean ± s.e.m. for 3 independent fractionations from separate animals.

c Immunohistology of 21DIV hippocampal neurons stained with anti-PSD-95 (red in merge) and anti-PrPC (6D11, green in merge) antibodies. Colocalization indicated by arrows. Scale bars, 4 µm.