Figure 2. Immunoprecipitation of CaV1.2 and AKAP79. (A) western blots of co-immunoprecipitations of CaV1.2-HA, calcium channel β3-flag, and AKAP79-myc in 1% CHAPS using FLAG sepharose beads. The anti-flag antibody identifies the β3-flag protein, while the AKAP79-myc protein is detected in the calcium channel complex indicating that AKAP79-myc binds to the calcium channel complex. (B) CaV1.2-HA is also immunoprecipitated by β3-flag protein as expected.