Figure 4. Interaction between the CaV1.2 C-terminal and II-III linker region. (A) In vitro binding of 6xHis II-III loop of CaV1.2 to immobilized CaV1.2 GST C-terminus. The western blot was probed using the anti-Xpress antibody. Grouping of images from different parts of the same gel have been made. (B) Binding between 6xHis II-III loop of CaV1.2 and CaV1.2 GST-C-terminal in the presence of increasing AKAP79 levels. Note that AKAP79 prevents the II-III linker C-terminus association. Bottom: Control blot to show that identical amounts of GST C-terminal fusion protein were used in the assay.