Fig. 10.
When ENaC is inhibited, Na+-K+-2Cl− cotransport blockade increases Cl− absorption. CCDs taken from Aldo-treated mice (treatment 1) were perfused with ANG II (10−8 M) in the bath. A and B: JCl and VT were measured in the absence (A) or presence (B) of benzamil (3 μM) in the luminal fluid. Solid lines display results from experiments where the vehicle was applied in the first period and benzamil was applied in the second period. Dashed lines indicate experiments where the order was reversed (i.e., benzamil was applied in the initial period and then removed in the second period). Open squares show means ± SE of data under each condition.