Fig. 11.
Effect of bumetanide on benzamil-sensitive JCl and VT. The effects of benzamil (3 μM) on JCl (left) and VT (right) were measured in CCDs from Aldo-treated mice (treatment 1) that were perfused with bumetanide (100 μM) with ANG II (10−8 M) present in the bath solution. Solid lines display results from experiments where the vehicle was applied in the first period and benzamil was applied in the second period. Dashed lines indicate experiments where the order was reversed (i.e., benzamil was applied in the initial period and then removed in the second period). Open squares show means ± SE of data under each condition.