Fig. 5.
Benzamil increases Cl− secretion. CCDs taken from mice that received a high-NaCl [high-salt (HS)] diet (treatment 3) were perfused in vitro in the absence of ANG II. Under basal conditions (vehicle), JCl was very low. With the application of benzamil to the perfusate, Cl− secretion was observed. Solid lines display results from experiments in which the vehicle was applied in the first period and benzamil was applied in the second period. Dashed lines indicate experiments where the order was reversed (i.e., benzamil was applied in the initial period and then removed in the second period). Open squares show means ± SE under each condition.