Fig. 7.

NKCC1 localizes to the basolateral regions of intercalated cells in the outer medullary collecting duct (OMCD) in Aldo-treated mice but not in vehicle-treated mice. Shown are sections of the outer medulla from mice given Aldo or vehicle (treatments 1 or 2) that were labeled for NKCC1. Areas in the boxes of A and B are shown at higher power in C and D. No NKCC1 labeling was noted in OMCDs taken from vehicle-treated mice (A and C). However, in mice given Aldo, NKCC1 label was observed in the basolateral regions of a minority cell type within the OMCD (B and D, arrows).