TABLE 1.
B. subtilis strains
| Strain | Genotype | Source or Ref. |
|---|---|---|
| 168CAa | Wild-type, trpC2 | Laboratory stock |
| BSB168b | Wild-type | trp+ derivative of Marburg 168 (64) |
| BBA9006 | PgapB-gfp/spec | 65 |
| GM1500 | trpC2 gapB′::pMUTIN2(ery) | 32 |
| GM1608 | trpC2 ytsJ′::pEC23-kan | 41 |
| GM2975 | Δyqfl::phleo | chr. DNA PS1632 (49) → BSB168 |
| GM2976 | Δyqfl::phleo, PgapB-gfp/spec | chr. DNA PS1632 (49) → BBA9006 |
| GTD107 | trpC2 gapB′::pMUTIN(ery) ytsJ′::pEC23-kan | chr. DNA GM1500 → GM1608 |
| PS1679 | trpC2 Δ (ccpN-yqfL)::phleo | 49 |
a Used strain for transcript analysis, enzyme assays, and 13C- and 2H-labeled experiments.
b Used strain for monitoring kinetics of PgapB promoter activity.