TABLE 2.

Physiological data of nitrogen starvation-induced resting B. subtilis wild-type and mutant cultures at 5.5 h after resuspension

Strain	Glucose uptake ratea	Specific production rates
		Acetoin	Citrate	Othersb
	mmol·g−1·h−1	mmol·g−1·h−1
Wild-typec	1.92	0.83	0.25	0.09
Wild-typed	1.99	0.82	0.28	0.09
gapBd	2.32	1.06	0.41	0.16
ytsJd	1.97	0.94	0.20	0.11
gapB/ytsJd	1.63	0.49	0.69	0.09

^a Errors were estimated from two independent wild-type experiments to be within 5% for glucose uptake and acetoin production rate, and within 10% for citrate and the other TCA cycle intermediates.

^b Shown are the summed secretion rates of the TCA cycle intermediates α-ketoglutarate, succinate, fumarate, and malate. Acetate and pyruvate were not produced.

^c Wild-type strain was grown in bioreactor. Based on the physiological rates and a CO₂ production rate of 6.04 mmol·g⁻¹·h⁻¹ in this bioreactor experiment, the carbon balance closed to 98%.

^d Strains were grown in shake flask cultures.