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. 2012 Jun 22;287(33):27601–27613. doi: 10.1074/jbc.M112.354670

TABLE 2.

Synthetic peptides prepared in this study

P3arv, reversed P3a peptide; P3am, mutated P3a peptide.

Blades B3B4-derived peptides
    P1 (621–640)a RGKMLLFSGRRLWRFDVKAQ
    P2 (638–657) KAQMVDPRSASEVDRMFPGV
    P3 (654–674) PGVPLDTHDVFQYREKAYFC
    P4 (672–691) YFCQDRFYWRVSSRSELNQV
    P5 (689–707) NQVDQVGYVTYDILQCPED

P3-derived peptides
    P3a FPGVPLDTHDVFQYREKb
    P3b VPLDTHDVFQb
    P3arv KERYQFVDHTDLPVGPF
    P3am FPGVPLATHAVFQYREKb

CS1, CS3 peptides
    CS1 LVTLPHPNLHGPEILDVPST
    CS3 TSGQQPSVGQQMIFEEHGFR

5(6)-Carboxyfluorescein-labeled peptides
    CF-P3a CF-FPGVPLDTHDVFQYREK
    CF-P3am CF-FPGVPLATHAVFQYREK
    CF-CS1 CF-LVTLPHPNLHGPEILDVPST
    CF-CS3 CF-TSGQQPSVGQQMIFEEHGFR

a Numbers in parenthesis indicate the position within proMMP-9 of the amino acid residues contained in each peptide.

b The two aspartate residues (D) mutated to alanine (A) are highlighted in bold.