FIGURE 2.

A30P α-synuclein aggregate formation upon high copy expression in yeast. Live-cell fluorescence microscopy of yeast cells expressing α-synuclein-KLID-GFP from high copy plasmids was compared with immunofluorescence of untagged α-synucleins. Yeast cells pre-grown to mid-log phase were induced in galactose-containing medium and examined for aggregates at 8 h of induction. GFP-expressing cells were used as control. Aggregate formation of untagged WT, A30P, and A53T α-synuclein was visualized by immunofluorescence. N-terminal eGFP-tagged α-synuclein via SAAAG linker and α-synuclein C-terminally fused to myeGFP was employed for further comparison. White arrows point at intracellular inclusions. Scale bar = 1 μm.