TABLE 1.
Yeast plasmids used in this study
| Plasmid | Description | Source |
|---|---|---|
| pME2795 | pRS426-GAL1-Promoter, CYC1-Terminator, URA3, 2 μm, pUC origin, AmpR | This study |
| pME3526 | pME2795 with GAL1::eGFP::SNCAWT (SAAAG linker) | This study |
| pME3527 | pME2795 with GAL1::eGFP::SNCAA30P (SAAAG linker) | This study |
| pME3528 | pME2795 with GAL1::eGFP::SNCAA53T (SAAAG linker) | This study |
| pME3759 | pME2795 with GFP | This study |
| pME3760 | pME2795 with GAL1::SNCAWT | This study |
| pME3761 | pME2795 with GAL1::SNCAA30P | This study |
| pME3762 | pME2795 with GAL1::SNCAA53T | This study |
| pME3941 | pME2795 with GAL1::SNCAA30P/A56P/A76P | This study |
| pME3763 | pME2795 with GAL1::SNCAWT::GFP (KLID linker) | This study |
| pME3764 | pME2795 with GAL1::SNCAA30P::GFP (KLID linker) | This study |
| pME3765 | pME2795 with GAL1::SNCAA53T::GFP (KLID linker) | This study |
| pME3942 | pME2795 with GAL1::SNCAA30P/A56P/A76P::GFP (KLID linker) | This study |
| pME3766 | pME2795 with GAL1::SNCAWT::myeGFP (AAAG linker) | This study |
| pME3943 | pME2795 with GAL1::SNCAWT::GFP (AAAG linker) | This study |
| pME3769 | pME2795 with GAL1::SNCAWT::myeGFP (no linker) | This study |
| pME3770 | pME2795 with GAL1::SNCAA30P::myeGFP (no linker) | This study |
| pME3771 | pME2795 with GAL1::SNCAA53T::myeGFP (no linker) | This study |
| pME3772 | pME2795 with GAL1::SNCAWT::mCherry (KLID linker) | This study |
| pME3773 | pME2795 with GAL1::SNCAWT::myeGFP (KLID linker) | This study |
| pME3774 | pRS306-GAL1-Promoter, CYC1-Terminator, URA3, integrative, pUC origin, AmpR | This study |
| pME3945 | pME3774 with SNCAWT::GFP (KLID linker) | This study |
| pME3946 | pME3774 with SNCAA30P::GFP (KLID linker) | This study |
| pME3947 | pME3774 with SNCAA53T::GFP (KLID linker) | This study |
| pME3948 | pCu416ATG8-GFP, Cu promoter | Ref. 32 |